Oppenheimer Research Fellow

I am a biophysicist working on super-resolution fluorescence microscopy in the Lee Lab. My current work focuses on estimating the orientation of single molecules using optical microscopes.

Before I joined the Lee Lab in October 2019, I studied bioengineering at the University of Antwerp (Belgium) and biophysics at KU Leuven (Belgium) during which I worked on the development of a novel method for spectral single-molecule localization microscopy in the group of Prof. Peter Dedecker.

Publications

SMOLM-LFM: ratiometric single molecule orientation without polarizers
AG Vesga, L Aleman-Castaneda, HS Heil, S Daly, E Bruggeman, SF Lee, S Brasselet, R Henriques
(2025)
Nano-org, a functional resource for single-molecule localisation microscopy data.
S Shirgill, DJ Nieves, JA Pike, MA Ahmed, H Abbott, MHH Baragilly, K Savoye, JD Worboys, KS Hazime, E Bruggeman, A Garcia, DJ Williamson, P Rubin-Delanchy, R Peters, DM Davis, R Henriques, SF Lee, DM Owen
Nature communications
(2025)
16
POLCAM: Instant molecular orientation microscopy for the life sciences
E Bruggeman, O Zhang, L-M Needham, M Körbel, S Daly, M Cheetham, R Peters, T Wu, AS Klymchenko, SJ Davis, EK Paluch, D Klenerman, MD Lew, K O'Holleran, SF Lee
Nat Methods
(2024)
21
dSTORM of actin in fixed HeLa cells v1
E Bruggeman, R peters
(2024)
Live-cell imaging of the plasma membrane of Jurkat T cells v1
E Bruggeman, M Körbel
(2024)
Preparation and imaging of lipid bilayer-coated silica microspheres v1
E Bruggeman
(2024)
Single-molecule orientation estimation using a polarisation camera
E Bruggeman
(2024)
High-density volumetric super-resolution microscopy
S Daly, J Ferreira Fernandes, E Bruggeman, A Handa, R Peters, S Benaissa, B Zhang, JS Beckwith, EW Sanders, RR Sims, D Klenerman, SJ Davis, K O’Holleran, SF Lee
Nature Communications
(2024)
15
TAB-PAINT imaging of alpha-synuclein fibrils using Nile Red v1
E Bruggeman
(2024)
Imaging single AF647 molecules immobilised in PVA on a cover glass v1
E Bruggeman
(2024)

Research Group