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Department of Chemistry

Portrait of tpjk2

We study the physical and chemical aspects of the behaviour of biopolymers and other soft systems. Much of our work has been focused on the physical aspects underlying the self-assembly of protein molecules. Self-organisation is the driving force generating complex matter in nature, and the process by which the machinery providing functionality in living systems is assembled. The goal of our research is to understand the physical and chemical factors which control the structures and dynamics of biomolecular assemblies, and the connections between the nanoscale characteristics of the component molecules and the physical properties of large-scale assemblies and their behaviour on a mesoscopic to macroscopic scale. The techniques used in our laboratory include biosensors, optical lithography, microfluidic devices and scanning probe microscopy and spectroscopy. We work both with natural and synthetic polymers and our interests range from fundamental chemical physics to technological applications in material science and molecular medicine.


Single molecule secondary structure determination of proteins through infrared absorption nanospectroscopy
FS Ruggeri, B Mannini, R Schmid, M Vendruscolo, TPJ Knowles
– Nat Commun
Continuous Flow Reactors from Microfluidic Compartmentalization of Enzymes within Inorganic Microparticles.
T Hakala, F Bialas, Z Toprakcioglu, B Bräuer, KN Baumann, A Levin, GJL Bernardes, CFW Becker, TPJ Knowles
– ACS Appl Mater Interfaces
Identification of on- and off-pathway oligomers in amyloid fibril formation
AJ Dear, G Meisl, A Šarić, TCT Michaels, M Kjaergaard, S Linse, TPJ Knowles
– Chemical Science
Rational design of a conformation-specific antibody for the quantification of Aβ oligomers
FA Aprile, P Sormanni, M Podpolny, S Chhangur, L-M Needham, FS Ruggeri, M Perni, R Limbocker, GT Heller, T Sneideris, T Scheidt, B Mannini, J Habchi, SF Lee, PC Salinas, TPJ Knowles, CM Dobson, M Vendruscolo
– Proceedings of the National Academy of Sciences
Microfluidic Templating of Spatially Inhomogeneous Protein Microgels.
Y Xu, RPB Jacquat, Y Shen, D Vigolo, D Morse, S Zhang, TPJ Knowles
– Small
Rapid Generation of Protein Condensate Phase Diagrams Using Combinatorial Droplet Microfluidics
W Arter, R Qi, G Krainer, T Welsh, Y Xu, PS George-Hyslop, S Alberti, TPJ Knowles
Direct digital sensing of proteins in solution through single-molecule optofluidics
G Krainer, K Saar, W Arter, RPB Jacquat, Q Peter, P Challa, C Taylor, D Klenerman, TPJ Knowles
Ultrastructural evidence for self-replication of Alzheimer-associated Aβ42 amyloid along the sides of fibrils
M Törnquist, R Cukalevski, U Weininger, G Meisl, TPJ Knowles, T Leiding, A Malmendal, M Akke, S Linse
– Proceedings of the National Academy of Sciences of the United States of America
Assessing motor-related phenotypes of Caenorhabditis elegans with the wide field-of-view nematode tracking platform
M Koopman, Q Peter, RI Seinstra, M Perni, M Vendruscolo, CM Dobson, TPJ Knowles, EAA Nollen
– Nature protocols
Kinetic diversity of amyloid oligomers.
AJ Dear, TCT Michaels, G Meisl, D Klenerman, S Wu, S Perrett, S Linse, CM Dobson, TPJ Knowles
– Proceedings of the National Academy of Sciences of the United States of America
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