skip to content

Department of Chemistry

 
Portrait of tpjk2

We study the physical and chemical aspects of the behaviour of biopolymers and other soft systems. Much of our work has been focused on the physical aspects underlying the self-assembly of protein molecules. Self-organisation is the driving force generating complex matter in nature, and the process by which the machinery providing functionality in living systems is assembled. The goal of our research is to understand the physical and chemical factors which control the structures and dynamics of biomolecular assemblies, and the connections between the nanoscale characteristics of the component molecules and the physical properties of large-scale assemblies and their behaviour on a mesoscopic to macroscopic scale. The techniques used in our laboratory include biosensors, optical lithography, microfluidic devices and scanning probe microscopy and spectroscopy. We work both with natural and synthetic polymers and our interests range from fundamental chemical physics to technological applications in material science and molecular medicine.

Publications

Trodusquemine enhances Aβ42 aggregation but suppresses its toxicity by displacing oligomers from cell membranes.
R Limbocker, S Chia, FS Ruggeri, M Perni, R Cascella, GT Heller, G Meisl, B Mannini, J Habchi, TCT Michaels, PK Challa, M Ahn, ST Casford, N Fernando, CK Xu, ND Kloss, SIA Cohen, JR Kumita, C Cecchi, M Zasloff, S Linse, TPJ Knowles, F Chiti, M Vendruscolo, CM Dobson
– Nature Communications
(2019)
10,
225
Soluble aggregates present in cerebrospinal fluid change in size and mechanism of toxicity during Alzheimer's disease progression
S De, DR Whiten, FS Ruggeri, C Hughes, M Rodrigues, DI Sideris, CG Taylor, FA Aprile, S Muyldermans, TPJ Knowles, M Vendruscolo, C Bryant, K Blennow, I Skoog, S Kern, H Zetterberg, D Klenerman
– Acta neuropathologica communications
(2019)
7,
120
Rapid two-dimensional characterisation of proteins in solution
KL Saar, Q Peter, T Müller, PK Challa, TW Herling, TPJ Knowles
– Microsystems & Nanoengineering
(2019)
5,
33
Different soluble aggregates of Aβ42 can give rise to cellular toxicity through different mechanisms
S De, DC Wirthensohn, P Flagmeier, C Hughes, FA Aprile, FS Ruggeri, DR Whiten, D Emin, Z Xia, JA Varela, P Sormanni, F Kundel, TPJ Knowles, CM Dobson, C Bryant, M Vendruscolo, D Klenerman
– Nature Communications
(2019)
10,
1541
Nucleation and Growth of Amino‐acid and Peptide Supramolecular Polymers through Liquid‐liquid Phase Separation
X Yan, C Yuan, A Levin, W Chen, R Xing, Q Zou, TW Herling, PK Challa, TPJ Knowles
– Angewandte Chemie (International ed. in English)
(2019)
Controllable coacervation of recombinantly produced spider silk protein using kosmotropic salts.
P Mohammadi, J Christopher, G Beaune, P Engelhardt, A Kamada, JVI Timonen, TPJ Knowles, M Penttila, MB Linder
– J Colloid Interface Sci
(2020)
560,
149
Modulating the Mechanical Performance of Macroscale Fibers through Shear-Induced Alignment and Assembly of Protein Nanofibrils
A Kamada, A Levin, Z Toprakcioglu, Y Shen, V Lutz-Bueno, KN Baumann, P Mohammadi, MB Linder, R Mezzenga, TPJ Knowles
– Small (Weinheim an der Bergstrasse, Germany)
(2019)
e1904190
RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether
Y-C Liao, MS Fernandopulle, G Wang, H Choi, L Hao, CM Drerup, R Patel, S Qamar, J Nixon-Abell, Y Shen, W Meadows, M Vendruscolo, TPJ Knowles, M Nelson, MA Czekalska, G Musteikyte, MA Gachechiladze, CA Stephens, HA Pasolli, LR Forrest, P St George-Hyslop, J Lippincott-Schwartz, ME Ward
– Cell
(2019)
179,
147
Characterising Individual Amyloid Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
FS Ruggeri, T Šneideris, S Chia, M Vendruscolo, TPJ Knowles
– Journal of Visualized Experiments
(2019)
(DOI: 10.3791/60108)
Scalable integration of nano-, and microfluidics with hybrid two-photon lithography
O Vanderpoorten, Q Peter, PK Challa, UF Keyser, J Baumberg, CF Kaminski, TPJ Knowles
– Microsystems and Nanoengineering
(2019)
5,
40
  • 1 of 31
  • >

Research Interest Groups

Telephone number

01223 336344

Email address

tpjk2@cam.ac.uk