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Yusuf Hamied Department of Chemistry

 

Our major research programme concerns the folding, stability and activity of proteins. We apply a broad multi-disciplinary approach that combines methods and ideas of molecular biology and physical-organic chemistry. We use techniques including protein engineering, DNA cloning, sequencing and mutagenesis, cell culture, gene and peptide synthesis, spectroscopy, rapid reaction techniques, multi-dimensional NMR (we have a 500, 600, 700 and an 800 MHz spectrometers) and x-ray protein crystallography.

Current major projects include: protein folding, misfolding and disease; drug discovery; and structure-activity relationships of proteins involved in cancer and disease.

Although now emeritus, I am still fully active in research with long term funding, including an MRC Programme Grant.

Publications

Φ‐Value Analysis of Protein Folding Transition States
N Ferguson, AR Fersht
(2011)
1,
81
Ancient watercourses and biogeography of the Sahara explain the peopling of the desert.
NA Drake, RM Blench, SJ Armitage, CS Bristow, KH White
– Proceedings of the National Academy of Sciences of the United States of America
(2010)
108,
458
Folding of the Pit1 homeodomain near the speed limit.
W Banachewicz, CM Johnson, AR Fersht
– Proceedings of the National Academy of Sciences of the United States of America
(2010)
108,
569
Electron microscopy studies on the quaternary structure of p53 reveal different binding modes for p53 tetramers in complex with DNA.
R Melero, S Rajagopalan, M Lázaro, AC Joerger, T Brandt, DB Veprintsev, G Lasso, D Gil, SHW Scheres, JM Carazo, AR Fersht, M Valle
– Proceedings of the National Academy of Sciences
(2010)
108,
557
A single-molecule characterization of p53 search on DNA
A Tafvizi, F Huang, AR Fersht, LA Mirny, AM van Oijen
– Proceedings of the National Academy of Sciences of the United States of America
(2010)
108,
563
Time-Resolved Small-Angle X-ray Scattering Study of the Folding Dynamics of Barnase
T Konuma, T Kimura, S Matsumoto, Y Goto, T Fujisawa, AR Fersht, S Takahashi
– Journal of molecular biology
(2010)
405,
1284
Kinetics of chain motions within a protein-folding intermediate
H Neuweiler, W Banachewicz, AR Fersht
– Proc Natl Acad Sci U S A
(2010)
107,
22106
Stabilization of mutant p53 via alkylation of cysteines and effects on DNA binding
JL Kaar, N Basse, AC Joerger, E Stephens, TJ Rutherford, AR Fersht
– Protein Sci
(2010)
19,
2267
Single-Molecule characterization of oligomerization kinetics and equilibria of the tumor suppressor p53.
S Rajagopalan, F Huang, AR Fersht
– Nucleic Acids Res
(2010)
39,
2294
Phosphotriesterase variants with high methylphosphonatase activity and strong negative trade-off against phosphotriesters.
L Briseño-Roa, CM Timperley, AD Griffiths, AR Fersht
– Protein Eng Des Sel
(2010)
24,
151
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