Dr Ezra Bruggeman

Oppenheimer Research Fellow

I am a biophysicist working on super-resolution fluorescence microscopy in the Lee Lab. My current work focuses on estimating the orientation of single molecules using optical microscopes.

Before I joined the Lee Lab in October 2019, I studied bioengineering at the University of Antwerp (Belgium) and biophysics at KU Leuven (Belgium) during which I worked on the development of a novel method for spectral single-molecule localization microscopy in the group of Prof. Peter Dedecker.

Publications

Imaging single SYTOX Orange molecules on a PLL-coated cover glass v1

E Bruggeman

(2024)

(doi: 10.17504/protocols.io.x54v9pbbmg3e/v1)

High-density volumetric super-resolution microscopy

S Daly, JF Fernandes, E Bruggeman, A Handa, R Peters, S Benaissa, B Zhang, JS Beckwith, EW Sanders, RR Sims, D Klenerman, SJ Davis, K O’Holleran, SF Lee

(2023)

(doi: 10.1101/2023.05.02.539032)

POLCAM: Instant molecular orientation microscopy for the life sciences

E Bruggeman, O Zhang, L-M Needham, M Körbel, S Daly, M Cheetham, R Peters, T Wu, AS Klymchenko, SJ Davis, EK Paluch, D Klenerman, MD Lew, K O’Holleran, SF Lee

(2023)

(doi: 10.1101/2023.02.07.527479)

Fast and artifact-free excitation multiplexing using synchronized image scanning

E Bruggeman, R Van den Eynde, B Amouroux, T Venneman, PV Berghe, M Müller, W Vandenberg, P Dedecker

(2023)

(doi: 10.1101/2023.02.07.527342)

resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.

EW Sanders, AR Carr, E Bruggeman, M Körbel, SI Benaissa, RF Donat, AM Santos, J McColl, K O'Holleran, D Klenerman, SJ Davis, SF Lee, A Ponjavic

Angewandte Chemie (International ed. in English)

(2022)

e202206919

(doi: 10.1002/anie.202206919)

resPAINT: Accelerating Volumetric Super‐Resolution Localisation Microscopy by Active Control of Probe Emission**

EW Sanders, AR Carr, E Bruggeman, M Körbel, SI Benaissa, RF Donat, AM Santos, J McColl, K O'Holleran, D Klenerman, SJ Davis, SF Lee, A Ponjavic

Angew Chem Weinheim Bergstr Ger

(2022)

134

e202206919

(doi: 10.1002/ange.202206919)

resPAINT: Accelerating volumetric super-resolution localisation microscopy by active control of probe emission

EW Sanders, AR Carr, E Bruggeman, M Koerbel, SI Benaissa, RF Donat, AM Santos, J McColl, K O’Holleran, D Klenerman, SJ Davis, SF Lee, A Ponjavic

(2022)

(doi: 10.1101/2022.04.14.488333)

Accelerating paint superresolution imaging by active probe control

EW Sanders, AR Carr, E Bruggeman, M Koerbel, AM Santos, JT McColl, K O'Holleran, D Klenerman, SJ Davis, A Ponjavic, SF Lee

Biophysical Journal

(2022)

121

140

(doi: 10.1016/j.bpj.2021.11.2025)

Quantification of the NA dependent change of shape in the image formation of a z-polarized fluorescent molecule using vectorial diffraction simulations

F Ströhl, E Bruggeman, CJ Rowlands, DL Wolfson, BS Ahluwalia

Microscopy Research and Technique

(2022)

2016

(doi: 10.1002/jemt.24060)

EAP45 association with budding HIV-1: Kinetics and domain requirements

B Meng, PP Vallejo Ramirez, KM Scherer, E Bruggeman, JC Kenyon, CF Kaminski, AM Lever

Traffic

(2021)

439

(doi: 10.1111/tra.12820)

Dr Ezra Bruggeman

Oppenheimer Research Fellow

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