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Yusuf Hamied Department of Chemistry

 

Our major research programme concerns the folding, stability and activity of proteins. We apply a broad multi-disciplinary approach that combines methods and ideas of molecular biology and physical-organic chemistry. We use techniques including protein engineering, DNA cloning, sequencing and mutagenesis, cell culture, gene and peptide synthesis, spectroscopy, rapid reaction techniques, multi-dimensional NMR (we have a 500, 600, 700 and an 800 MHz spectrometers) and x-ray protein crystallography.

Current major projects include: protein folding, misfolding and disease; drug discovery; and structure-activity relationships of proteins involved in cancer and disease.

Although now emeritus, I am still fully active in research with long term funding, including an MRC Programme Grant.

Publications

Experimental assignment of the structure of the transition state for the association of barnase and barstar.
C Frisch, AR Fersht, G Schreiber
– Journal of molecular biology
(2001)
308,
69
Structure of the C-terminal sterile alpha-motif (SAM) domain of human p73 alpha.
WK Wang, M Bycroft, NW Foster, AM Buckle, AR Fersht, YW Chen
– Acta Crystallographica Section D, Structural Biology
(2001)
57,
545
Protein folding from a highly disordered denatured state: the folding pathway of chymotrypsin inhibitor 2 at atomic resolution.
SL Kazmirski, KB Wong, SM Freund, YJ Tan, AR Fersht, V Daggett
– Proc Natl Acad Sci U S A
(2001)
98,
4349
Ligand-independent assembly of recombinant human CD1 by using oxidative refolding chromatography
MM Altamirano, A Woolfson, A Donda, A Shamshiev, L BriseƱo-Roa, NW Foster, DB Veprintsev, G De Libero, AR Fersht, C Milstein
– Proceedings of the National Academy of Sciences of the United States of America
(2001)
98,
3288
The binding of bis-ANS to the isolated GroEL apical domain fragment induces the formation of a folding intermediate with increased hydrophobic surface not observed in tetradecameric GroEL.
AL Smoot, M Panda, BT Brazil, AM Buckle, AR Fersht, PM Horowitz
– Biochemistry
(2001)
40,
4484
Preface
CM Dobson, RJ Ellis, AR Fersht
– Philosophical transactions of the Royal Society of London. Series B, Biological sciences
(2001)
356,
129
Preface
CM Dobson, R John Ellis, AR Fersht
– Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences
(2001)
356,
129
Protein folding and unfolding in microseconds to nanoseconds by experiment and simulation (vol 97, pg 13518, 2000)
U Mayor, CM Johnson, V Daggett, AR Fersht
– PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2001)
98,
777
Structure on the Move
WA Hendrickson, CI Branden, AR Fersht
– Structure
(2001)
9,
r1
From minichaperone to GroEL 1: Information on GroEL-polypeptide interactions from crystal packing of minichaperones
Q Wang, AM Buckle, AR Fersht
– Journal of Molecular Biology
(2000)
304,
873
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