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400 MHz Avance III HD Spectrometer - 'Lagavulin' - Room B25

This spectrometer is now available for open-access use once again after its reinstallation. Book together with Tobermory on Clustermarket during the Covid pandemic.

The 400 MHz open-access spectrometer in B25 is one of our Avance III HD spectrometers with a Nanobay console.  It has a BBO Smart Probe which can observe 1H and a wide range of nuclei on its broadband channel.  We have configured the instrument to run 1H, 7Li, 11B, 13C, 19F, 27Al and 31P acquisitions.  This probe exhibits greater sensitivity on both the Proton and Broadband channels compared to the old style QNP probes.  The sample changer is one of the latest SampleXpress types, which improves throughput on this busy open-access spectrometer which runs a four figure amount of spectra each month.  The basic standard experiments that it will run are:

1. Quick Proton - Daytime experiment

2. Quick 19F and Proton - Daytime experiment

3. Quick 31P and Proton - Daytime experiment

4. Quick Proton, Carbon and DEPT135 - Daytime experiment

5. Quick Proton and COSY - Daytime experiment

6. Quick 11B - Daytime experiment

7. 1H, 13C, DEPT135, COSY, HSQC, and HMBC - Overnight experiment (requires at least 10mg of sample for best results)

8. Longer Fluorine and Proton coupled Fluorine + Proton - Overnight experiment

9. Longer Phosphorus and Proton coupled Phosphorus + Proton - Overnight experiment

10. Longer 13C and DEPT135 + Proton - Overnight experiment

11. NOESY (mixing time 0.6sec) and COSY - Overnight experiment

Other experiments are available if you require them, please consult the drop down list on the submission PC or the guide to experiments on the poster above it.

By default there will be no print outs of spectra, as we have found in the past that most of the paper output is not picked up and is subsequently recycled. If you require a printed copy of your data you should processs the data yourself using TopSpin which is available free of charge for academic use - see the links on our home page.

All data will be emailed to you if you type your email address into the comments box, regardless of experiment chosen.

The experiments are queued in order of submission for daytime and overnight experiments and the machine automatically works through the list in turn.  Daytime experiments will run overnight if there is spare time.  The overnight period runs from 11pm to 8am which offers late-workers the chance to get one of the quick daytime experiments run before they leave for the night.

When submitting samples to the instrument would all users please stick to the following convention:

Group Code followed crsid and then /, followed by your personal identifying code. For example DRS-ABC25/ABC5_100_Fr1 or SB-XY321/XIAOYUN-1-35-2

By following these rules it makes things much easier when preparing the usage lists at the end of each month.

All the data is archived and backed up on the Analytical file-server storage system. Please go to the NMR Data page for full details of how to access your spectra.

Please remember to collect your tubes promptly after they have been run.  Tubes left in the room are cleared every Tuesday morning around 8:30am and can be found in the labelled beaker in the fume hood in B28. Tubes that subsequently remain uncollected from our room for a further week are likely to be washed up or offered free of charge to other researchers on a first-come first-served basis.

The image below shows the system in its old home in B12, coupled to the 400 MHz magnet that was once part of Prof. Steve Ley's 400 instrument.