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Professor Tuomas Knowles

Portrait of tpjk2

We study the physical and chemical aspects of the behaviour of biopolymers and other soft systems. Much of our work has been focused on the physical aspects underlying the self-assembly of protein molecules. Self-organisation is the driving force generating complex matter in nature, and the process by which the machinery providing functionality in living systems is assembled. The goal of our research is to understand the physical and chemical factors which control the structures and dynamics of biomolecular assemblies, and the connections between the nanoscale characteristics of the component molecules and the physical properties of large-scale assemblies and their behaviour on a mesoscopic to macroscopic scale. The techniques used in our laboratory include biosensors, optical lithography, microfluidic devices and scanning probe microscopy and spectroscopy. We work both with natural and synthetic polymers and our interests range from fundamental chemical physics to technological applications in material science and molecular medicine.

Selected publications

An analytical solution to the kinetics of breakable filament assembly, Science, 326, 1533 (2009)

Role of intermolecular forces in defining material properties of protein nanofibrils, Science 318, 1900 (2007)

Publications

Kinetics of fragmentation and dissociation of two-strand protein filaments: Coarse-grained simulations and experiments.
A Zaccone, I Terentjev, TW Herling, TPJ Knowles, A Aleksandrova, EM Terentjev
– J. Chem. Phys.
(2016)
145,
105101
Mutations associated with familial Parkinson's disease alter the initiation and amplification steps of α-synuclein aggregation.
P Flagmeier, G Meisl, M Vendruscolo, TPJ Knowles, CM Dobson, AK Buell, C Galvagnion
– Proceedings of the National Academy of Sciences of the United States of America
(2016)
113,
10328
Protein Aggregate-Ligand Binding Assays Based on Microfluidic Diffusional Separation.
Y Zhang, AK Buell, T Müller, E De Genst, J Benesch, CM Dobson, TPJ Knowles
– Chembiochem
(2016)
Synthesis of Nonequilibrium Supramolecular Peptide Polymers on a Microfluidic Platform
TO Mason, TCT Michaels, A Levin, E Gazit, CM Dobson, AK Buell, TPJ Knowles
– Journal of the American Chemical Society
(2016)
138,
9589
Controlling the Physical Dimensions of Peptide Nanotubes by Supramolecular Polymer Coassembly
L Adler-Abramovich, P Marco, ZA Arnon, RCG Creasey, TCT Michaels, A Levin, DJ Scurr, CJ Roberts, TPJ Knowles, SJB Tendler, E Gazit
– ACS Nano
(2016)
10,
7436
Quantifying Measurement Fluctuations from Stochastic Surface Processes on Sensors with Heterogeneous Sensitivity
J Charmet, TCT Michaels, R Daly, A Prasad, P Thiruvenkathanathan, RS Langley, TPJ Knowles, AA Seshia
– Physical Review Applied
(2016)
5,
ARTN 064016
Fluctuations in the Kinetics of Linear Protein Self-Assembly
TCT Michaels, AJ Dear, JB Kirkegaard, KL Saar, DA Weitz, TPJ Knowles
– Phys Rev Lett
(2016)
116,
258103
Particle-Based Monte-Carlo Simulations of Steady-State Mass Transport at Intermediate Péclet Numbers
T Müller, P Arosio, L Rajah, SIA Cohen, EV Yates, M Vendruscolo, CM Dobson, TPJ Knowles
– International Journal of Nonlinear Sciences and Numerical Simulation
(2016)
175
Amyloid Fibrils as Building Blocks for Natural and Artificial Functional Materials
TPJ Knowles, R Mezzenga
– Adv Mater
(2016)
28,
6546
A Microfluidic Platform for Real-Time Detection and Quantification of Protein-Ligand Interactions.
TW Herling, DJ O'Connell, MC Bauer, J Persson, U Weininger, TPJ Knowles, S Linse
– Biophys J
(2016)
110,
1957
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Research Group

Research Interest Groups

Telephone number

01223 763845 (shared)
01223 336344

Email address

tpjk2@cam.ac.uk