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Department of Chemistry

 

I am an EPSRC post-doctoral research fellow at the research group of Prof. Tuomas Knowles and a Fellow-Elect at St. John's College. My research focusses on developing new methods for probing the behaviour of biological molecules and systems, in particular proteins. Proteins are the executive molecules of life that through diverse set of highly integrated and tightly regulated interactions set the molecular basis for our well-being. Yet the diverse and dynamic nature of proteins has made it challenging to devise methods that could be effectively used for understanding the roles of proteins in human health and disease. As part of my current research, I am briniging state-of-the-art micron and nano scale fabrication and flow engineering approaches that I developed during my PhD research together with single molecule optical tools to develop methods that would open up new insight into protein behavior down to a digital level. In particular, one of my goals is to develop an eplorative probe-free platform for single cell proteomic analysis.

Publications

Analysis of αB-crystallin polydispersity in solution through native microfluidic electrophoresis.
MA Wright, FS Ruggeri, KL Saar, PK Challa, JLP Benesch, TPJ Knowles
– The Analyst
(2019)
144,
4413
Quaternization of Vinyl/Alkynyl Pyridine Enables Ultrafast Cysteine-Selective Protein Modification and Charge Modulation.
MJ Matos, CD Navo, T Hakala, X Ferhati, A Guerreiro, D Hartmann, B Bernardim, KL Saar, I Compañón, F Corzana, TPJ Knowles, G Jiménez-Osés, GJL Bernardes
– Angew Chem Int Ed Engl
(2019)
58,
6640
Combining Affinity Selection and Specific Ion Mobility for Microchip Protein Sensing.
WE Arter, J Charmet, J Kong, KL Saar, TW Herling, T Müller, UF Keyser, TPJ Knowles
– Analytical Chemistry
(2018)
90,
10302
Massively parallel C elegans tracking provides multi-dimensional fingerprints for phenotypic discovery
M Perni, PK Challa, JB Kirkegaard, R Limbocker, M Koopman, MC Hardenberg, P Sormanni, T Müller, KL Saar, LWY Roode, J Habchi, G Vecchi, N Fernando, S Casford, EAA Nollen, M Vendruscolo, CM Dobson, TPJ Knowles
– Journal of Neuroscience Methods
(2018)
306,
57
Enhancing the Resolution of Micro Free Flow Electrophoresis through Spatially Controlled Sample Injection
KL Saar, T Müller, J Charmet, PK Challa, TPJ Knowles
– Analytical Chemistry
(2018)
90,
8998
Real-Time Intrinsic Fluorescence Visualization and Sizing of Proteins and Protein Complexes in Microfluidic Devices.
PK Challa, Q Peter, MA Wright, Y Zhang, KL Saar, JA Carozza, JLP Benesch, TPJ Knowles
– Anal Chem
(2018)
90,
3849
On-chip measurements of protein unfolding from direct observations of micron-scale diffusion
Y Zhang, EV Yates, L Hong, KL Saar, G Meisl, CM Dobson, TPJ Knowles
– Chem Sci
(2018)
9,
3503
Microfluidic approaches for probing amyloid assembly and behaviour.
TW Herling, A Levin, KL Saar, CM Dobson, TPJ Knowles
– Lab on a Chip
(2018)
18,
999
Enhancing power density of biophotovoltaics by decoupling storage and power delivery
KL Saar, P Bombelli, DJ Lea-Smith, T Call, EM Aro, T Müller, CJ Howe, TPJ Knowles
– Nature Energy
(2018)
3,
75
Mechanism of biosurfactant adsorption to oil/water interfaces from millisecond scale tensiometry measurements.
L Kong, KL Saar, R Jacquat, L Hong, A Levin, H Gang, R Ye, B Mu, TPJ Knowles
– Interface focus
(2017)
7,
20170013
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Research Group

Telephone number

01223 336359

Email address

kls78@cam.ac.uk