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Ezra Bruggeman

Research Associate

I am a biophysicist working on super-resolution fluorescence microscopy in the Lee Lab. My current work focuses on estimating the orientation of single molecules using optical microscopes.

Before I joined the Lee Lab in October 2019, I studied bioengineering at the University of Antwerp (Belgium) and biophysics at KU Leuven (Belgium) during which I worked on the development of a novel method for spectral single-molecule localization microscopy in the group of Prof. Peter Dedecker.

Selected Publications 

POLCAM: Instant molecular orientation microscopy for the life sciences
E Bruggeman, O Zhang, L-M Needham, M Körbel, S Daly, M Cheetham, R Peters, T Wu, A Klymchenko, S Davis, E Paluch, D Klenerman, M Lew, K O’Holleran, S Lee (2023)
High-density volumetric super-resolution microscopy
S Daly, J Ferreira Fernandes, E Bruggeman, A Handa, R Peters, S Benaissa, B Zhang, JS Beckwith, EW Sanders, RR Sims, D Klenerman, SJ Davis, K O’Holleran, SF Lee – Nature Communications (2024) 15, 1940
resPAINT: Accelerating volumetric super-resolution localisation microscopy by active control of probe emission
E Sanders, A Carr, E Bruggeman, M Koerbel, S Benaissa, R Donat, AM Santos, J McColl, K O’Holleran, D Klenerman, S Davis, S Lee, A Ponjavic (2022)
Single Molecule Light Field Microscopy
RR Sims, SA Rehman, MO Lenz, SI Benaissa, E Bruggeman, A Clark, EW Sanders, A Ponjavic, L Muresan, SF Lee, K O’Holleran – Optica (2020) 7, 1065

Publications

High-density volumetric super-resolution microscopy
S Daly, JF Fernandes, E Bruggeman, A Handa, R Peters, S Benaissa, B Zhang, J Beckwith, E Sanders, R Sims, D Klenerman, S Davis, K O’Holleran, S Lee
(2023)
(doi: 10.1101/2023.05.02.539032)
Fast and artifact-free excitation multiplexing using synchronized image scanning
E Bruggeman, R Van den Eynde, B Amouroux, T Venneman, P Vanden Berghe, M Müller, W Vandenberg, P Dedecker
(2023)
(doi: 10.1101/2023.02.07.527342)
POLCAM: Instant molecular orientation microscopy for the life sciences
E Bruggeman, O Zhang, L-M Needham, M Körbel, S Daly, M Cheetham, R Peters, T Wu, A Klymchenko, S Davis, E Paluch, D Klenerman, M Lew, K O’Holleran, S Lee
(2023)
(doi: 10.1101/2023.02.07.527479)
resPAINT: Accelerating Volumetric Super-Resolution Localisation Microscopy by Active Control of Probe Emission.
EW Sanders, AR Carr, E Bruggeman, M Körbel, SI Benaissa, RF Donat, AM Santos, J McColl, K O'Holleran, D Klenerman, SJ Davis, SF Lee, A Ponjavic
– Angewandte Chemie (Weinheim an der Bergstrasse, Germany)
(2022)
134,
e202206919
(doi: 10.1002/ange.202206919)
Quantification of the NA dependent change of shape in the image formation of a z-polarized fluorescent molecule using vectorial diffraction simulations.
F Ströhl, E Bruggeman, CJ Rowlands, DL Wolfson, BS Ahluwalia
– Microscopy research and technique
(2022)
85,
2016
(doi: 10.1002/jemt.24060)
EAP45 association with budding HIV‐1: Kinetics and domain requirements
B Meng, PP Vallejo Ramirez, KM Scherer, E Bruggeman, JC Kenyon, CF Kaminski, AM Lever
– Traffic (Copenhagen, Denmark)
(2021)
22,
439
(doi: 10.1111/tra.12820)
Single Molecule Light Field Microscopy
RR Sims, SA Rehman, MO Lenz, SI Benaissa, E Bruggeman, A Clark, EW Sanders, A Ponjavic, L Muresan, SF Lee, K O’Holleran
– Optica
(2020)
7,
1065
(doi: 10.1364/optica.397172)
Distinct domain requirements for EAP45 in HIV budding, late endosomal recruitment, and cytokinesis
B Meng, P Vallejo Ramirez, K Scherer, E Bruggeman, J Kenyon, C Kaminski, A Lever
– bioRxiv
(2020)
2020.05.23.112607
(doi: 10.1101/2020.05.23.112607)
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Postgraduate student

College

Lucy Cavendish College

    Study at Cambridge

    About the University

    Research at Cambridge