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Ezra Bruggeman

Research Associate

I am a biophysicist working on super-resolution fluorescence microscopy in the Lee Lab. My current work focuses on estimating the orientation of single molecules using optical microscopes.

Before I joined the Lee Lab in October 2019, I studied bioengineering at the University of Antwerp (Belgium) and biophysics at KU Leuven (Belgium) during which I worked on the development of a novel method for spectral single-molecule localization microscopy in the group of Prof. Peter Dedecker.

Selected Publications 

POLCAM: Instant molecular orientation microscopy for the life sciences
E Bruggeman, O Zhang, L-M Needham, M Körbel, S Daly, M Cheetham, R Peters, T Wu, A Klymchenko, S Davis, E Paluch, D Klenerman, M Lew, K O’Holleran, S Lee (2023)
High-density volumetric super-resolution microscopy
S Daly, J Ferreira Fernandes, E Bruggeman, A Handa, R Peters, S Benaissa, B Zhang, JS Beckwith, EW Sanders, RR Sims, D Klenerman, SJ Davis, K O’Holleran, SF Lee – Nature Communications (2024) 15, 1940
resPAINT: Accelerating volumetric super-resolution localisation microscopy by active control of probe emission
E Sanders, A Carr, E Bruggeman, M Koerbel, S Benaissa, R Donat, AM Santos, J McColl, K O’Holleran, D Klenerman, S Davis, S Lee, A Ponjavic (2022)
Single molecule light field microscopy
RR Sims, SA Rehman, MO Lenz, SI Benaissa, E Bruggeman, A Clark, EW Sanders, A Ponjavic, L Muresan, SF Lee, K O’Holleran – Optica (2020) 7, 1065

Publications

POLCAM: Instant molecular orientation microscopy for the life sciences
E Bruggeman, O Zhang, L-M Needham, M Körbel, S Daly, M Cheetham, R Peters, T Wu, AS Klymchenko, SJ Davis, EK Paluch, D Klenerman, MD Lew, K O'Holleran, SF Lee
– Nature methods
(2024)
21,
1873
(doi: 10.1038/s41592-024-02382-8)
dSTORM of actin in fixed HeLa cells v1
E Bruggeman, R peters
(2024)
(doi: 10.17504/protocols.io.q26g714j8gwz/v1)
Live-cell imaging of the plasma membrane of Jurkat T cells v1
E Bruggeman, M Körbel
(2024)
(doi: 10.17504/protocols.io.kxygxyzxzl8j/v1)
Preparation and imaging of lipid bilayer-coated silica microspheres v1
E Bruggeman
(2024)
(doi: 10.17504/protocols.io.rm7vzjy2rlx1/v1)
Single-molecule orientation estimation using a polarisation camera
E Bruggeman
(2024)
(doi: 10.17863/CAM.112690)
High-density volumetric super-resolution microscopy
S Daly, J Ferreira Fernandes, E Bruggeman, A Handa, R Peters, S Benaissa, B Zhang, JS Beckwith, EW Sanders, RR Sims, D Klenerman, SJ Davis, K O'Holleran, SF Lee
– Nature communications
(2024)
15,
1940
(doi: 10.1038/s41467-024-45828-5)
High-density volumetric super-resolution microscopy
S Daly, J Ferreira Fernandes, E Bruggeman, A Handa, R Peters, S Benaissa, B Zhang, JS Beckwith, EW Sanders, RR Sims, D Klenerman, SJ Davis, K O’Holleran, SF Lee
– Nature Communications
(2024)
15,
1940
(doi: 10.1038/s41467-024-45828-5)
TAB-PAINT imaging of alpha-synuclein fibrils using Nile Red v1
E Bruggeman
(2024)
(doi: 10.17504/protocols.io.n2bvj31mwlk5/v1)
Imaging single SYTOX Orange molecules on a PLL-coated cover glass v1
E Bruggeman
(2024)
(doi: 10.17504/protocols.io.x54v9pbbmg3e/v1)
Imaging single AF647 molecules immobilised in PVA on a cover glass v1
E Bruggeman
(2024)
(doi: 10.17504/protocols.io.eq2lyj5bplx9/v1)
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Postgraduate student

College

Lucy Cavendish College

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    Research at Cambridge