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The Vion allows trained users to acquire accurate mass data in either polarity using electrospray ionisation. Tandem mass spectrometry and IMS (Ion mobility) data can also be acquired.

IMS allows the user to determine the collision cross-section of a molecule, the separation of ions with equal mass and charge but different collisional cross section, which is also another confirmation of compound identity and an indicator of compound structure. Theoretical values can be calculated using molecular modelling and compared directly with the experimentally determined values yielding useful information on the structures and shapes of large proteins/protein complexes, peptides, organometallic complexes and small molecules.

Tandem or MS/MS acquisitions allow the user to investigate fragmentation pathways leading to structural information.