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500 MHz TCI Cryoprobe Spectrometer - 'Glenlivet' - Room B19

During the COVID-19 pandemic Glenlivet and Glengrant are combined as one room (B19) available to book on Clustermarket in 45 minute blocks of time starting on the hour from 0800 to 2200. You can book up to four of these blocks in a day. If you book consecutive blocks the 15 minutes changeover time is available to work right through free of charge. Otherwise you must finish your experiments at 45 minutes, sanitise the room and vacate to leave as much time as possible before the next user. You can use either or both of the instruments during your session for the price of just one, so with a bit of careful planning and creative working with your colleagues one person can achieve a lot of results for little cost. Email to book the overnight slot from 2300 to 0800 and 2300 Saturday to 0800 Monday as we will control the bookings for these periods.

This instrument is now using Bruker's "loan" TCI Cryoprobe to replace our broken one. This will mean new pulse lengths and power handling settings. DO NOT use or import any "old" datasets back into your working directories, as this may damage the probe (at OUR cost!!). Please use the *.tci parameter sets that Duncan has created. On your first log-in, drag the gramicidin-s dataset from the D:/data/<yourgroup>/nmr directory into TopSpin to start off correctly, then load a *.tci parameter set. Please see Duncan or Andrew for help with this if required.

One of our two 500 MHz instruments in B19, the Avance 500 TCI Cryoprobe system enhances our abilities in the area of protein chemistry. With its Triple Resonance Inverse (TCI) Automatic Tuning and Matching (ATM) cryoprobe, CHN combination experiments are possible. Chemistry of Health research groups make use of this instrument, together with our highest field 700 MHz spectrometer for these types of studies.  However, the suitability for experiments such as 3D TOCSY-NOESY, WATERGATE presaturation and other techniques together with a 60 position sample changer, mean that this instrument is also in demand from those groups who are conducting research with smaller molecules, and those using water as a primary solvent.

This instrument is equipped with a 5mm TCI ATM cryoprobe with Z-Gradients.  TCI probes are specially designed to allow a reasonable sensitivity to carbon nuclei as well as nitrogen and proton, whereas the older TXI style probe had very poor carbon performance.  We still advise however that carbon experiments on samples of low concentration should be carried out on the Service DCH 500 cryoprobe in B19.

The instrument is run by the NMR Facility team as required, and also by individually trained group users.  A wide variety of experiments are available for use including 1H, COSY, HSQC, HMBC, TOCSY, ROESY and NOESY, together with Gradient nOe, WATERGATE 1H and presaturation versions of 2D experiments.  There are also parameter sets for the major groups of experiments used by our protein chemists - we will be able to advise on the best people to talk to if you require more help in this area, and can arrange for Bruker specialists to visit if something very complex requires setting up.  Most users will find the basic library of programs cover their needs with minor adjustments to suit.  Hard and soft, as well as decoupler pulse calibration is needed to get the most out of certain samples however.  Please talk to the NMR Facility team if you need assistance - we can usually help and get your experiment running quickly and safely.

This instrument runs with an online booking system which can be found here.