400 MHz Avance III HD Smart Probe Spectrometer - 'Laphroaig' - Room B22
CURRENTLY RUNNING WITH A SPARE PROBE OFFERING 1H & 19F OBSERVE EXPERIMENTS ONLY
The 400 MHz spectrometer in B22 is one of our open access walk-up instruments. This spectrometer has an Avance III HD Nanobay electronics console, Smart Probe and SampleXpress sample changer in conjunction with a recently refurbished magnet. This instrument is one of our busiest - running thousands of samples each year. The Smart Probe is able to deliver higher sensitivity than the previous QNP probe on both the 1H and Broadband channel. The broadband channel means that it is possible to observe a wide range of X-nuclei under full automation, although in regular operation we will be offering 1H, 13C, 19F and 31P acquisitions. The spectrometer is controlled by a bespoke program, and submission of samples takes place on the separate computer. The basic experiments that this spectrometer will run are:
1. Quick Proton - Daytime experiment
2. Quick 19F and Proton - Daytime experiment
3. Quick 31P and Proton - Daytime experiment
4. Quick Proton, Carbon and DEPT135 - Daytime experiment
5. Quick Proton and COSY - Daytime experiment
6. 1H, 13C, DEPT135, COSY, HSQC and HMBC - Overnight experiment (requires at least 10mg of sample for best results)
7. Longer Fluorine and Proton coupled Fluorine + Proton - Overnight experiment
8. Longer Phosphorus and Proton coupled Phosphorus + Proton - Overnight experiment
9. Longer 13C and DEPT135 + Proton - Overnight experiment
10. NOESY (mixing time 0.6sec) and COSY - Overnight experiment
Other experiments are available if you require them, please consult the drop down list on the submission PC.
By default there are no print outs of spectra, as we have found in the past that most of the paper output is not picked up and is subsequently recycled. If you require a printed copy of your data you should process the data yourself using TopSpin which is available free of charge for academic use - see the links on our home page.
All data will be emailed to you if you type your email address into the comments box, regardless of experiment chosen.
The experiments are queued in order of submission for daytime and overnight experiments and the machine automatically works through the list in turn. Daytime experiments will run overnight if there is spare time. The overnight period runs from 7pm to 8am. Users may wish to consider running their samples on the other open-access spectrometer (Lagavulin) once the overnight period commences, as it is set up to allow daytime experiments until midnight.
When submitting samples to the instrument would all users please stick to the following convention:
Group Code followed by crsid then /, followed by your personal identifying code. For example MJG-ABC32/ABC10_Fr2 or ER-SB123/SIMON-11-1a
By following these rules it makes things much easier when preparing the usage lists at the end of each month.
All the data is archived and backed up on the Analytical file-server storage system. Please go to the NMR Data page for full details of how to access your spectra.
Please remember to collect your tubes promptly after they have been run. Tubes left in the room are cleared every Tuesday morning around 8:30am and can be found in the labelled beaker in the cold room lobby (CRL). If they subsequently remain uncollected from the CRL for a few weeks then they are likely to be washed up or offered free of charge to other researchers on a first-come first-served basis.
