Research in my group can be divided into two areas, although these share a common theme of engineering metal protein interactions in novel ways.
One goal is to engineer novel proteins and polypeptide based assemblies that can be used in molecular electronic devices and nanotechnology in general. This involves understanding, at a fundamental level, how metal cofactors, particularly heme, is delivered to proteins in vivo and, in the case of c-type cytochromes, how heme is covalently attached to protein. It also involves understanding how functional protein units can be assembled into larger nanoscale assemblies that gain function through the proximity of the constituent monomers.
The other goal is to explore the interaction of 4d and 5d tranistion metals with proteins, particularly as a possible route to finding novel medicinal compounds. Specifically, Ruthenium organometallic complexes have shown some potential as anti cancer compounds, but little is understood about how the chemistry of Ruthenium interacts with biomolecules.
Analysis of the bimolecular reduction of ferricytochrome c by ferrocytochrome b5 through mutagenesis and molecular modelling.
JG GUILLEMETTE, PD BARKER, LD ELTIS, TP LO, M SMITH, GD BRAYER, AG MAUK - Biochimie (
1994)
76, 592
(DOI:
10.1016/0300-9084(94)90136-8)
Transmutation of a heme protein.
PD BARKER, JC FERRER, M MYLRAJAN, TM LOEHR, R FENG, Y KONISHI, WD FUNK, RTA MACGILLIVRAY, AG MAUK - Proceedings of the National Academy of Sciences of the United States of America (
1993)
90, 6542
(DOI:
10.1073/pnas.90.14.6542)
Effects of charged amino acid mutations on the bimolecular kinetics of reduction of yeast iso-1-ferricytochrome c by bovine ferrocytochrome b5
SH NORTHRUP, KA THOMASSON, CM MILLER, PD BARKER, LD ELTIS, JG GUILLEMETTE, SC INGLIS, AG MAUK - Biochemistry (
1993)
32, 6613
(DOI:
10.1021/bi00077a014)
ELECTROCHEMICAL ADSORPTION OF PEPTIDES AS PROMOTERS OF DIRECT ELECTRON-TRANSFER IN SOLUTIONS WITH DIFFERENT PH ELECTROCHEMICAL STUDIES
AY SAFRONOV, HAO HILL, PD BARKER, K DIGLERIA - RUSSIAN ELECTROCHEMISTRY (1993) 29, 811
pH-linked conformational regulation of a metalloprotein oxidation-reduction equilibrium: Electrochemical analysis of the alkaline form of cytochrome c
PD BARKER, AG MAUK - Journal of the American Chemical Society (
1992)
114, 3619
(DOI:
10.1021/ja00036a006)
Proton linkage of complex formation between cytochrome c and cytochrome b5: electrostatic consequences of protein-protein interactions.
MR MAUK, PD BARKER, AG MAUK - Biochemistry (
1991)
30, 9873
(DOI:
10.1021/bi00105a010)
DIRECT ELECTROCHEMISTRY OF 2 GENETICALLY DISTINCT FLAVODOXINS ISOLATED FROM AZOTOBACTER-CHROOCOCCUM GROWN UNDER NITROGEN-FIXING CONDITIONS
S BAGBY, PD BARKER, HAO HILL, GS SANGHERA, B DUNBAR, GA ASHBY, RR EADY, RNF THORNELEY - Biochemical Journal (1991) 277 ( Pt 2), 313
REDUCTION OF HORSE HEART FERRICYTOCHROME-C BY BOVINE LIVER FERROCYTOCHROME-B5 - EXPERIMENTAL AND THEORETICAL-ANALYSIS
LD ELTIS, RG HERBERT, PD BARKER, AG MAUK, SH NORTHRUP - Biochemistry (
1991)
30, 3663
(DOI:
10.1021/bi00229a011)
PROTON TITRATION CURVE OF YEAST ISO-1-FERRICYTOCHROME-C - ELECTROSTATIC AND CONFORMATIONAL EFFECTS OF POINT MUTATIONS
PD BARKER, MR MAUK, AG MAUK - Biochemistry (
1991)
30, 2377
(DOI:
10.1021/bi00223a012)
ELECTROCHEMICAL, KINETIC, AND CIRCULAR DICHROIC CONSEQUENCES OF MUTATIONS AT POSITION-82 OF YEAST ISO-1-CYTOCHROME-C
SP RAFFERTY, LL PEARCE, PD BARKER, JG GUILLEMETTE, CM KAY, M SMITH, AG MAUK - Biochemistry (
1990)
29, 9365
(DOI:
10.1021/bi00492a009)
